Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup
Summary: Aquaporins (AQPs) are water channels embedded in the cell membrane that are critical in maintaining water homeostasis.We describe a protocol for screenprotector determining the water permeation capacity of AQPs reconstituted into proteoliposomes.Using a stopped-flow setup, AQP embedded in proteoliposomes are exposed to an osmogenic gradien